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Enhanced survival of transplanted human adipose-derived stem cells by co-delivery with liposomal apoptosome inhibitor in fibrin gel matrix
Gayong Shim, Saewon Im, Sangbin Lee, Joo Yeon Park, Jinyoung Kim, Hyerim Jin, Soondong Lee, Irang Im, Dae-Duk Kim, Seong Who Kim, Taik Jong Lee, Jin Sup Eom, Tac-Ghee Yi, Sun Uk Song, Youngro Byun, Yu-Kyoung Oh
European Journal of Pharmaceutics and Biopharmaceutics (SCIE)
85 (3)
줄기세포의 생착율증강을 위한 apoptosis저해제의 리포좀제제화 연구

To improve the survival of transplanted human adipose-derived stem cells (ADSCs), a liposome preparation containing the apoptosome inhibitor, NS3694, was formulated and co-delivered with ADSCs in fibrin gel scaffolds. Liposomes provided enhanced effect on ADSC proliferation in vitro as compared to free drug. Exposure of ADSCs to liposomal NS3694 for 7 days did not affect the surface marker expression profile. NS3694 encapsulated in negatively charged liposomes composed of phosphatidylcholine, phosphatidylglycerol, and cholesterol was evaluated in vivo following subcutaneous transplantation in mice. Survival of ADSCs co-delivered with liposomal NS3694 was significantly higher than that of untreated ADSCs or ADSCs treated with free NS3694 or empty liposomes. An immunohistochemical analysis revealed a higher number of human nucleus-positive cells after treatment with liposomal NS3694 than following treatment with free NS3694. Similarly, liposomal NS3694 significantly enhanced survival of transplanted ADSCs in rabbits compared to other treatments. Taken together, our results indicate the potential of liposomal NS3694 co-delivered with ADSCs using fibrin gel systems as an in vivo-survival enhancer.